Fig. 1: MEG3 was downregulated in NAFLD. | Cell Death Discovery

Fig. 1: MEG3 was downregulated in NAFLD.

From: LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease

Fig. 1

A–C Primary hepatocytes were treated with FFA for 0, 12, and 24 h, respectively. A Lipid accumulation was examined by Oil Red O staining. B Intracellular triglycerides level was measured by colorimetric assay. C MEG3 expression was examined by RT-qPCR. D–G Mice were fed with HFD for 12 weeks to induce NAFLD. Mice fed with normal diet were used as control. HE staining (D) and Oil Red O staining (E) of liver tissues from indicated groups. F Triglyceride level from the liver tissue was measured. G MEG3 expression in liver tissue was examined by RT-qPCR. H RT-qPCR was performed to measure MEG3 expression from normal livers (n = 10), livers with steatosis (n = 15), and those with fibrosis (n = 15). I RT-qPCR was performed to measure MEG3 expression from patients diagnosed with simple steatosis (n = 9) and those from patients with NASH (n = 6). *P < 0.05, **P < 0.01.

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