Fig. 4: SIRT6 mediated the protection of MEG3 on FFA-challenged hepatocytes. | Cell Death Discovery

Fig. 4: SIRT6 mediated the protection of MEG3 on FFA-challenged hepatocytes.

From: LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease

Fig. 4

A The expression of SIRT6 was measured by Western blot in indicated hepatocytes. B Binding of EZH2 and H3K27me3 to the promoter of SIRT6 was detected by ChIP assay. IgG was used as the negative control. C SIRT6 expression in indicated hepatocytes was measured by RT-qPCR (upper panel) and Western blot (lower panel). In indicated hepatocytes, Oil Red O staining (D), triglyceride assay (E), mRNA (F) and protein (G) of indicated lipogenesis-related genes, and expressions of pro-inflammatory TNF-α, IL-6, IL-1β, and CCL2 (H) were performed. *P < 0.05, **P < 0.01, ***P < 0.001.

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