Table 1 Sequences for primers used for RT-qPCR assay.
Gene | Species | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
MEG3 | Mouse | CACAGAAGACGAAGAGCTGGA | GGTAGAGGTGCACAGCAGGT |
TTTTGTGCCCAAGGCTCCTGGA | AGGGACTCAAGGAGCCAGGTTA | ||
EZH2 | Mouse | CATACGCTCTTCTGTCGACGATG | ACACTGTGGTCCACAAGGCTTG |
GACCTCTGTCTTACTTGTGGAGC | CGTCAGATGGTGCCAGCAATAG | ||
SIRT6 | Mouse | CAGTACGTCAGAGACACGGTTG | GTCCAGAATGGTGTCTCTCAGC |
TGGCAGTCTTCCAGTGTGGTGT | CGCTCTCAAAGGTGGTGTCGAA | ||
CD36 | Mouse | GGACATTGAGATTCTTTTCCTCTG | GCAAAGGCATTGGCTGGAAGAAC |
FAS | Mouse | CTGCGATTCTCCTGGCTGTGAA | CAACAACCATAGGCGATTTCTGG |
ACC1 | Mouse | GTTCTGTTGGACAACGCCTTCAC | GGAGTCACAGAAGCAGCCCATT |
SCD1 | Mouse | GCAAGCTCTACACCTGCCTCTT | CGTGCCTTGTAAGTTCTGTGGC |
SREBP-1c | Mouse | CGACTACATCCGCTTCTTGCAG | CCTCCATAGACACATCTGTGCC |
PPARα | Mouse | ACCACTACGGAGTTCACGCATG | GAATCTTGCAGCTCCGATCACAC |
CPT1A | Mouse | GGCATAAACGCAGAGCATTCCTG | CAGTGTCCATCCTCTGAGTAGC |
TNF-α | Mouse | GGTGCCTATGTCTCAGCCTCTT | GCCATAGAACTGATGAGAGGGAG |
IL-6 | Mouse | TACCACTTCACAAGTCGGAGGC | CTGCAAGTGCATCATCGTTGTTC |
IL-1β | Mouse | TGGACCTTCCAGGATGAGGACA | GTTCATCTCGGAGCCTGTAGTG |
CCL-2 | Mouse | GCTACAAGAGGATCACCAGCAG | GTCTGGACCCATTCCTTCTTGG |
GAPDH | Mouse | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
