Fig. 6: DMF inhibits macrophage polarization to M1 phenotype after treatment with ferrous myoglobin. | Cell Death Discovery

Fig. 6: DMF inhibits macrophage polarization to M1 phenotype after treatment with ferrous myoglobin.

From: Myoglobin promotes macrophage polarization to M1 type and pyroptosis via the RIG-I/Caspase1/GSDMD signaling pathway in CS-AKI

Fig. 6

a–d qPCR analyses iNOS, CD86, TNF-α, IL-6 expression in NC, Mb, Mb + DMF, and DMF group. e, f WB and flow cytometry analyse iNOS expression in NC, Mb, Mb+DMF, and DMF group. g, h Representative confocal microscopy images of cells in NC, Mb, Mb + DMF, and DMF group stained for iNOS and CD86 (Scale bars: 20 μm). For statistical analysis, two-factor ANOVA followed by Tukey’s method of multiple comparisons used in (a–d). Data are expressed as mean ± SD, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001.

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