Fig. 1: Mouse and cell models of atrial fibrosis exhibit abundant SPP1 expression. | Cell Death Discovery

Fig. 1: Mouse and cell models of atrial fibrosis exhibit abundant SPP1 expression.

From: RETRACTED ARTICLE: Anti-fibrotic mechanism of SPP1 knockdown in atrial fibrosis associates with inhibited mitochondrial DNA damage and TGF-β/SREBP2/PCSK9 signaling

Fig. 1

A HE staining of pathological changes of atrial tissues in normal and Ang II-treated mice. B Masson’s trichrome staining of atrial fibrosis degree in normal and Ang II-treated mice. Collagen fibers were in blue and muscle fibers were in red. C IHC analysis of collagen I and collagen III proteins in atrial tissues of normal and Ang II-treated mice. D CCK-8 detection of cell viability in control and Ang II-induced HL-1 cells. E TUNEL detection of apoptosis of control and Ang II-induced HL-1 cells. F Western blot analysis of collagen I and collagen III proteins in control and Ang II-induced HL-1 cells. G A volcano plot of differential genes in the GSE27133 dataset. The abscissa indicates −log10 (p-value), and the ordinate indicates log2FC value. H Venn diagram of the DEGs and atrial fibrosis-related genes. I A heat map of key candidate gene expression. The color scale from blue to red indicates the increasing expression value. J SPP1 expression in atrial fibrosis samples in the GSE27133 dataset. K RT-qPCR detection of SPP1 expression in atrial tissues of normal and Ang II-treated mice. L RT-qPCR detection of SPP1 expression in control and Ang II-induced HL-1 cells. *p < 0.05, compared with the normal mice or control HL-1 cells. All experiments were repeated three times independently. n = 10 for mice in each group.

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