Fig. 2: SPP1 silencing delays atrial fibrosis. | Cell Death Discovery

Fig. 2: SPP1 silencing delays atrial fibrosis.

From: RETRACTED ARTICLE: Anti-fibrotic mechanism of SPP1 knockdown in atrial fibrosis associates with inhibited mitochondrial DNA damage and TGF-β/SREBP2/PCSK9 signaling

Fig. 2

A RT-qPCR detection of SPP1 expression in HL-1 cells treated with sh-SPP1-1 or sh-SPP1-2. B CCK-8 detection of cell viability in HL-1 cells treated with sh-SPP1. C TUNEL detection of apoptosis of HL-1 cells treated with sh-SPP1. D Western blot analysis of collagen I and collagen III proteins in HL-1 cells treated with sh-SPP1. E HE staining of pathological changes of atrial tissues of mice treated with sh-SPP1. F Masson’s trichrome staining of atrial fibrosis degree in mice treated with sh-SPP1. G IHC analysis of collagen I and collagen III proteins in atrial tissues of mice treated with sh-SPP1. *p < 0.05, compared with the Ang II-induced HL-1 cells or mice treated with sh-NC. All experiments were repeated three times independently. n = 10 for mice in each group.

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