Fig. 3: SPP1 enhances atrial fibrosis by increasing the expression of TGF-β. | Cell Death Discovery

Fig. 3: SPP1 enhances atrial fibrosis by increasing the expression of TGF-β.

From: RETRACTED ARTICLE: Anti-fibrotic mechanism of SPP1 knockdown in atrial fibrosis associates with inhibited mitochondrial DNA damage and TGF-β/SREBP2/PCSK9 signaling

Fig. 3

A Venn diagram of co-expressed genes of SPP1 in the Coxpresdb database and atrial fibrosis-related genes. B KEGG enrichment analysis on candidate genes. The abscissa represents GeneRatio. C Correlation between SPP1 and TGF-β in heart tissues analyzed by the Chipbase v2.0 database (r = 0.6641, p-value = 3.65e−49). D RT-qPCR detection of TGF-β expression in atrial tissues of normal and Ang II-treated mice. E RT-qPCR detection of TGF-β expression in control and Ang II-induced HL-1 cells. F RT-qPCR detection of TGF-β expression in HL-1 cells treated with sh-SPP1. G Western blot analysis of TGF-β protein in HL-1 cells treated with sh-SPP1. H RT-qPCR detection of TGF-β expression in HL-1 cells treated with oe-TGF-β. I Western blot analysis of TGF-β protein in HL-1 cells treated with oe-TGF-β. J RT-qPCR detection of SPP1 and TGF-β expression in HL-1 cells treated with sh-SPP1 or combined with oe-TGF-β. K CCK-8 detection of cell viability in HL-1 cells treated with sh-SPP1 or combined with oe-TGF-β. L TUNEL detection of apoptosis of HL-1 cells treated with sh-SPP1 or combined with oe-TGF-β. M Western blot analysis of collagen I and collagen III proteins in HL-1 cells treated with sh-SPP1 alone or in combination with oe-TGF-β. N HE staining of pathological changes of atrial tissues of Ang II-induced mice treated with sh-SPP1 or combined with oe-TGF-β. O Masson’s trichrome staining of atrial fibrosis degree in Ang II-induced mice treated with sh-SPP1 or combined with oe-TGF-β. P IHC analysis of collagen I and collagen III proteins in atrial tissues of Ang II-induced mice treated with sh-SPP1 or combined with oe-TGF-β. *p < 0.05, compared with the normal mice, control HL-1 cells, Ang II-induced HL-1 cells treated with sh-NC, oe-NC or sh-NC + oe-NC or Ang II-induced mice treated with sh-NC + oe-NC. # p < 0.05, compared with the Ang II-induced HL-1 cells or mice treated with sh-SPP1 + oe-NC. All experiments were repeated three times independently. n = 10 for mice in each group.

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