Fig. 6: NSUN5 recruits TRAP1 to modify FTH1 and FTL.
From: The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells
A Silver-stained gel showing a protein band among the pulldown products of bone marrow-derived stem cells (BMSCs) expressing NSUN5 that was absent in those of NSUN5-depleted cells; western blot analysis confirmed TRAP1 enrichment in the former group. B Detection of NSUN5/TRAP1 binding complexes by RNA immunoprecipitation (RIP). *p < 0.05. C Immunofluorescence analysis confirmed that NSUN5 and TRAP1 protein co-localized in the cytoplasm of BMSCs. *p < 0.05. D Interaction between TRAP1 and FTH1/FTL revealed by co-immunoprecipitation (co-IP). E, F NSUN5 knockdown reduced the amount of precipitated FTH1/FTL, indicating that the interaction between TRAP1 and FTH1/FTL was abolished. G–I NSUN5 silencing did not affect the mRNA and protein levels of TRAP1 and vice versa, *p < 0.05 (B).
