Fig. 5: CTHRC1 could affect integrinβ3/FAK signaling. | Cell Death Discovery

Fig. 5: CTHRC1 could affect integrinβ3/FAK signaling.

From: Long noncoding RNA LINC00518 contributes to proliferation and metastasis in lung adenocarcinoma via the miR-335-3p/CTHRC1 Axis

Fig. 5

A The correlation of CTHRC1 and integrin αVβ3 from the TCGA database. B Pathway analysis and functional analysis was separately according to TCGA database and DAVID database. C Western blotting showed that integrin β3, p-FAK, FAK, MMP-9, and CyclinD1 protein levels were regulated by CTHRC1. D Co-immunoprecipitation of CTHRC1 and integrin β3 in LUAD cells exposed that endogenous integrin β3 in LUAD cells was immunoprecipitated by CTHRC1 antibody. E The co-expression between CTHRCI and integrin β3 was shown by IHC of sections from the patients (scale bar, 100 μm, 20 μm). F CCK-8 assays were applied to detect the cell with VS6063 (5 μmol/L) had notably lower capability of cell proliferation than the control cells with DMSO (5 μmol/L). G The IC50 of A549 and H1299 cells, which inhibited miR-335-3p. H The IC50 of A549 and H1299 cells, which inhibited miR-335-3p. I CCK-8 assay showed the proliferation of A549/H1299 cells that inhibited miR-335-3p transfection with VS6063 (5 μmol/L). I Transwell assays showed that miR-335-3p inhibited cells migration and invasion following transfection. J Western blotting analysis showed that CTHRC1 and p-FAK protein levels were induced by a FAK-inhibitor when miR-335-3p was inhibited. All results indicates SD. (*P < 0.05, **P < 0.01, ***P < 0.001).

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