Fig. 2: Necroptosis inhibitors can rescue cell death caused by knockdown of HABON under hypoxia.
From: LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening
A–F The expression of HABON was knockdown in SMMC-7721, Huh7 and hepG2 cells. The viable cells were counted after cultured under normoxia or hypoxia (1% O2) and treated with apoptosis inhibitor (20 μM Z-VAD-fmk) or necroptosis inhibitor (20 μM Nec-1, 5 μM GSK872) for different time. Three independent experiments, two-tailed Student’s t-test. G–I Flow cytometry was used to detect cell death after PI staining following treated with apoptosis or necroptosis inhibitor for 24 h. And the statistics of PI positive cells were shown. Error bars stand for mean ± SD. Three independent experiments, two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.
