Fig. 5: Damage of mitochondrial function resulted from knockdown of HABON under hypoxia was mediated by mPTP opening. | Cell Death Discovery

Fig. 5: Damage of mitochondrial function resulted from knockdown of HABON under hypoxia was mediated by mPTP opening.

From: LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening

Fig. 5

A Transfect siHABON or its control into liver cancer cells, and cells were cultured under normoxia or hypoxia with or without CsA (5 μM) for 24 h. Perform mPTP assay in each group of hepG2 cells and the opening of mPTP was visualized and measured by confocal microscopy. B–D Measure the cellular ATP content. Three independent experiments, two-tailed Student’s t-test. E, F Quantification of ROS content in each group of SMMC-771 cells by DCFH-DA flow cytometry. And the statistics of mean fluorescence were shown. G–I Mitochondrial membrane potential in each group of samples were measured by TMRM flow cytometry and relative fluorescence intensity was calculated. Error bars stand for mean ± SD. Three independent experiments, two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

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