Fig. 6: Necroptosis caused from knockdown of HABON under hypoxia was mediated by mPTP opening.
From: LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening
A, B Expression levels of necroptosis marker RIPK1, phospho-RIPK1 (Ser166), MLKL, and phospho-MLKL (Ser358) were measured by western blot following by knockdown HABON expression in liver cancer cells with or without hypoxia treatment. C, D The expression of HABON was knock-down in SMMC-7721 cells. The viable cells were measured after cultured under normoxia or hypoxia (1% O2) and treated with mPTP inhibitor (5 μM CsA) for different time. Three independent experiments, two-tailed Student’s t-test. E, F Flow cytometry was used to detect cell death of SMMC-771 and Huh7 cells after PI staining following treated with mPTP inhibitor (5 μM CsA) for 24 h. The statistics of PI positive cells were shown in G, H. Error bars stand for mean ± SD. Three independent experiments, two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. I The mechanism schematic of HABON involved in the regulation of necroptosis induced by hypoxia.
