Fig. 1: SNHG12 regulates the expression pattern of E2F7 through miRNA-dependent mechanism. | Cell Death Discovery

Fig. 1: SNHG12 regulates the expression pattern of E2F7 through miRNA-dependent mechanism.

From: Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12

Fig. 1

A The target binding sites of SNHG12 and miR-129-5p predicted by the RNA22 website. B The target binding sites of E2F7 and miR-129-5p predicted by the starbase website. C The expression box plot of E2F7 in STAD, the gray box represents the expression in normal samples, and the red box the expression in GC samples. D Interaction between SNHG12 and E2F7 in HPMCs detected using RIP assay. E WT and MUT binding sites between SNHG12 and miR-129-5p as well as between miR-129-5p and E2F7. F Luciferase activity analyzed using dual-luciferase reporter gene assay after HEK-293T cells were co-transfected with WT or MUT of SNHG12 or E2F7, miR-129-5p mimic or NC. G The co-localization of Cy3-miR-129-5p (red) and FITC-SNHG12 (green) in HPMCs cells measured using FISH (scale bar = 25 μm), and the nucleus was stained with DAPI (blue). H Direct binding between SNHG12 or E2F7 and miR-129-5p in HPMCs detected by RNA pull-down assay. I miR-129-5p expression pattern in HPMCs evaluated by RT-qPCR. J E2F7 protein expression pattern in HPMCs measured by western blot analysis. p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The experiment was conducted three times independently.

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