Fig. 3: EVs deliver SNHG12 to HPMCs. | Cell Death Discovery

Fig. 3: EVs deliver SNHG12 to HPMCs.

From: Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12

Fig. 3

A The morphology of EVs observed using TEM (scale bar = 100 nm). B EV diameter distribution measured using NTA. C The expression patterns of protein markers of EVs (Alix, TSG101, CD9, and Calnexin) detected by western blot analysis. D The admission of NCI-N87-EVs labeled with fluorescent PKH67 into HPMCs (PKH67 labeled EVs were green, DAPI stained nuclei blue, scale bar = 25 μm) observed by confocal laser scanning microscope through z-sections and orthogonal views analysis. E The expression pattern of SNHG12 after EVs were treated with RNase and Triton X100 detected using RT-qPCR. F The enter of FITC-labeled SNHG12 (green) into HPMCs via Dil-labeled EVs (red) observed by confocal laser scanning microscope through z-sections and orthogonal views analysis (DAPI: blue, scale bar = 25 µm). G The SNHG12 expression pattern in HPMCs detected by RT-qPCR after co-culture of HPMCs and NCI-N87-EVs. p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The experiment was conducted three times independently.

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