Fig. 4: Effects of EVs-SNHG12 from GC cells on apoptosis and MMT of HPMCs. | Cell Death Discovery

Fig. 4: Effects of EVs-SNHG12 from GC cells on apoptosis and MMT of HPMCs.

From: Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12

Fig. 4

A SNHG12 expression pattern in NCI-N87 cells and NCI-N87-EVs measured using RT-qPCR. B SNHG12 expression pattern in HPMCs after co-culture detected using RT-qPCR. C Cell viability of HPMCs after co-culture monitored using CCK-8. D The apoptosis of HPMCs after co-culture examined using flow cytometry. E The expression patterns of cleaved PARP and caspase-3 in HPMCs after co-culture determined by western blot analysis. F HPMC morphology observed by an inverted microscope (left, scale bar = 100 µm) and the proportion of abnormal cells (right). G The expression patterns of ZO-1 and Vimentin in HPMCs after co-culture measured using western blot analysis. H The expression patterns of ZO-1 and Vimentin in HPMCs after co-culture measured using immunofluorescence. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The experiment was conducted three times independently.

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