Fig. 5: EVs-SNHG12 regulates the miR-129-5p/E2F7/MAPK/ERK axis to affect HPMC apoptosis and MMT. | Cell Death Discovery

Fig. 5: EVs-SNHG12 regulates the miR-129-5p/E2F7/MAPK/ERK axis to affect HPMC apoptosis and MMT.

From: Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12

Fig. 5

A miR-129-5p expression pattern in HPMCs treated with EVs measured using RT-qPCR. B The expression patterns of E2F7, p/t-MEK, and p/t-ERK in HPMCs treated with EVs detected using western blot analysis. C Apoptosis of HPMCs after co-culture examined using flow cytometry. D The expression patterns of cleaved PARP and caspase-3 in HPMCs after co-culture examined by western blot analysis. E The expression patterns of ZO-1 and Vimentin in HPMCs after co-culture determined using western blot analysis. F The expression patterns of ZO-1 and Vimentin in HPMCs after co-culture determined using immunofluorescence. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The experiment was conducted three times independently.

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