Fig. 3: ZJU-37 promotes OPC proliferation in vitro with higher efficacy than Nec-1s. | Cell Death Discovery

Fig. 3: ZJU-37 promotes OPC proliferation in vitro with higher efficacy than Nec-1s.

From: Inhibition of RIPK1 by ZJU-37 promotes oligodendrocyte progenitor proliferation and remyelination via NF-κB pathway

Fig. 3

A Schematic diagram of the experiment for testing OPC proliferation in vitro. B Representative fluorescence images of proliferating primary rat OPCs treated with DMSO, Nec-1s (5 μM) or ZJU-37 (5 μM) for 36 h. Proliferating OPCs was visualized by Ki67 (red) and Olig2 (green). Scale bars, 50 μm. Quantification of proliferating OPCs over (C) time and (D) concentration of compounds (two-way repeated ANOVA, Sidak’s post hoc test, n = 3 plate wells). E Schematic diagram of the experiment for testing OPC differentiation in vitro. F Representative fluorescence images of differentiated primary rat OPCs treated with DMSO, Nec-1s or ZJU-37 for 48 h. Differentiated mature oligodendrocyte was visualized by MBP (red) and Olig2 (green). Scale bars, 50 μm. Quantification of differentiated OPC over (G) time and (H) drug concentration (two-way repeated ANOVA, Sidak’s post hoc test, n = 3 plate wells). All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance.

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