Fig. 4: ZJU-37 promotes OPC proliferation within the demyelination lesion in vivo. | Cell Death Discovery

Fig. 4: ZJU-37 promotes OPC proliferation within the demyelination lesion in vivo.

From: Inhibition of RIPK1 by ZJU-37 promotes oligodendrocyte progenitor proliferation and remyelination via NF-κB pathway

Fig. 4

A Schematic diagram of the experimental design. B Representative fluorescence images of proliferating OPCs (arrows, Olig2+Ki67+ cells) within the demyelination region (dotted line) at 5 dpl. Scale bars, 50 μm. C–E Quantification of (C) oligodendrocyte lineage cell numbers, (D) proliferating OPCs numbers and (E) proportion of proliferating OPCs in all oligodendrocyte lineage cells within the demyelination region at 5 dpl (two-tailed t test, n = 4 mice in the vehicle group, n = 5 mice in the ZJU-37 group). F Representative images of differentiated oligodendrocytes (arrows, Olig2+CC1+ cells) within the demyelination region at 10 dpl. DAPI (blue), Olig2 (green), CC1 (red). Scale bars, 50 μm. Quantification of (G) oligodendrocyte lineage cell numbers, (H) differentiated oligodendrocytes numbers and (I) proportion of differentiated oligodendrocytes in all oligodendrocyte lineage cells within the lesion region at 10 dpl (two-tailed t test, n = 5 mice in the vehicle group, n = 6 mice in the ZJU-37 group). All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance.

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