Fig. 5: ZJU-37 enhances remyelination in vivo. | Cell Death Discovery

Fig. 5: ZJU-37 enhances remyelination in vivo.

From: Inhibition of RIPK1 by ZJU-37 promotes oligodendrocyte progenitor proliferation and remyelination via NF-κB pathway

Fig. 5

A Schematic diagram of the experimental design. B Representative transmission electron micrographs within the demyelination region at 10 dpl. Scale bars, 1 μm. C–F Quantification of (C) remyelinated axons (two-tailed t test), (D) G-Ratio (two-tailed t test), (E) individual G-Ratio distribution (linear regression) and (F) myelin pathology level (two-way repeated ANOVA, Sidak’s post hoc test) within the demyelination region at 10 dpl (n = 5 mice). G Experimental design to test remyelination in vivo. H Representative electron micrographs within the demyelination region at 21 dpl. Scale bars, 1 μm (upper row), 500 nm (lower row). I–L Quantification of (I) remyelinated axons (two-tails t test), (J) G-Ratio (two-tailed t test), (K) individual G-Ratio distribution (linear regression) and (L) myelin pathology level (two-way repeated ANOVA, Sidak’s post hoc test) within the demyelination region at 21 dpl (n = 6 mice). M Quantification of remyelinated axons within the demyelination region at 3 dpl and 21 dpl (two-way repeated ANOVA, Sidak’s post hoc test). N Experimental design to test the effect of ZJU-37 on remyelination after completion of demyelination (ZJU-37 treatment started on the third day post LPC injection when demyelination has finished). O Representative transmission electron micrographs within the demyelination region at 21 dpl. P–S Quantification of (P) the remyelinated axons (two-tailed t test), (Q) G-Ratio (two-tailed t test), (R) individual G-Ratio distribution (linear regression) and (S) the myelin pathology level (two-way repeated ANOVA, Sidak’s post hoc test) within the demyelination region at 21 dpl (n = 5 mice). All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance.

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