Fig. 6: ZJU-37 promotes OPC proliferation and enhances remyelination by inhibiting RIPK1 kinase activity. | Cell Death Discovery

Fig. 6: ZJU-37 promotes OPC proliferation and enhances remyelination by inhibiting RIPK1 kinase activity.

From: Inhibition of RIPK1 by ZJU-37 promotes oligodendrocyte progenitor proliferation and remyelination via NF-κB pathway

Fig. 6

A Schematic diagram of the experimental design. B Representative fluorescence images of the proliferating OPCs within the demyelination region at 5 dpl. Scale bars, 50 μm. C–E Quantification of (C) the oligodendrocyte lineage cell numbers, (D) the proliferating OPCs numbers and (E) the proportion of proliferating OPCs in all oligodendrocyte lineage cells within the lesion region at 5 dpl (one-way ANOVA, Tukey’s post hoc test, n = 5 mice in the WT group, n = 4 mice in the RIPK1D138N group, n = 7 mice in the RIPK1D138N vehicle and RIPK1D138N ZJU-37 group). F Representative transmission electron micrographs within the demyelination region at 21 dpl. Scale bars, 1 μm (upper row), 500 nm (lower row). G–J Quantification of (G) the remyelinated axons (one-way ANOVA, Tukey’s post hoc test), (H) G-Ratio (one-way ANOVA, Tukey’s post hoc test), (I) individual G-Ratio distribution (linear regression) and (J) the myelin pathology level (two-way repeated ANOVA, Sidak’s post hoc test) within the demyelination region at 21 dpl (n = 5 mice per group). All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance.

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