Fig. 2: Veratridine induces expression of UBXN2A in vivo. | Cell Death Discovery

Fig. 2: Veratridine induces expression of UBXN2A in vivo.

From: Pre-clinical safety and therapeutic efficacy of a plant-based alkaloid in a human colon cancer xenograft model

Fig. 2

A Mice were treated with control (ethanol 0.01%) or 0.1 mg/kg VTD QOD IP for 30 days, and intestine was collected at endpoint. B, C Extracted small and large intestine tissues were subjected to qRT-PCR. Significant elevation of UBXN2A RNA in response to VTD treatment (N = 6 per treatment, *P < 0.05) occurred in the large intestine. The small intestine showed a non-significant elevation of UBXN2A in response to VTD (N = 6 per treatment, P = 0.08). D, E Tissue lysates of the large intestine from 4 control and 4 VTD treated mice were subjected to WB (Panel D). VTD treatment significantly enhanced the protein level of UBXN2A in large intestine tissues, confirming the three-fold increased UBXN2A RNA level in the presence of VTD (Panel E, N = 4 per treatment, dots represent individual animal expression, *P < 0.05).

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