Fig. 4: Acute and repeated exposure to VTD does not impact behavior in mice.

A Experimental timeline for mice treated with control or 0.1 mg/kg VTD QOD IP for 30 days and evaluated for behavioral impacts. B, C Results from an open field test to evaluate locomotor behavior following acute and repeated exposure to VTD. No treatment effects were found in total distance traveled (Panel B) or overall velocity (Panel C) within the open field chamber. Results are expressed as mean ± SEM (N = 8 per group, P > 0.05 for all comparisons). D, E Results from rotarod and grid hang grip tests evaluating motor coordination, balance, and limb strength following acute and repeated exposure to VTD. No treatment effects were found in total time on rotarod (Panel D) or grid hang grip strength as expressed as a percent of baseline (Panel E). Results are expressed as mean ± SEM (N = 8 per group, P > 0.05 for all comparisons). F, G Results from mechanical nociception response evaluating pain threshold and novel object recognition evaluating short-term memory behavior following acute and repeated exposure to VTD. No treatment effects were found in the mechanical nociception response (Panel F) or short-term memory behavior exhibited as the discrimination index of a novel object (Panel G). Results are expressed as mean ± SEM (N = 8 per group, P > 0.05 for all comparisons).