Fig. 2: miR-4731-5p suppresses the glycolysis, EMT, and migration and invasion of MCF-7 cells. | Cell Death Discovery

Fig. 2: miR-4731-5p suppresses the glycolysis, EMT, and migration and invasion of MCF-7 cells.

From: Tumor suppressive role of microRNA-4731-5p in breast cancer through reduction of PAICS-induced FAK phosphorylation

Fig. 2

A RT-qPCR was used to detect the expression of miR-4731-5p in clinical samples (n = 50). B RT-qPCR was used to detect the expression of miR-4731-5p in breast cancer cells (MDA-MB-453, MCF-7, and MDA-MB-231) and breast epithelial cells. C RT-qPCR was used to detect the expression of miR-4731-5p after miR-4731-5p mimic treatment. D The effect of miR-4731-5p on glycolysis of breast cancer cells. E The effect of miR-4731-5p on glucose and lactic acid content of breast cancer cells. F RT-qPCR was used to detect the expression of glycolysis-related genes PKM2, GLUT1, and EMT-related markers Vimentin and E-Cadherin. G Western blot analysis was used to detect the expression of glycolysis-related genes PKM2, GLUT1, and EMT-related markers Vimentin and E-Cadherin. H Immunofluorescence was used to detect the expression of EMT-related markers Vimentin and E-Cadherin, scale bar = 25 μm. I Detection of cell migration by scratch test after miR-4731-5p mimic treatment. J Detection of cell invasion by Transwell assay after miR-4731-5p mimic treatment. Measurement data were expressed as mean ± standard deviation. Comparison between two groups was performed through an independent t test. Analysis among multiple groups was conducted by one-way ANOVA followed by Tukey’s post hoc test. * p < 0.05 compared with adjacent normal tissues, MCF10A cell, or mimic NC treatment.

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