Fig. 2: Increased mitophagy and neuronal injury after hypoxia treatment.
From: Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice
A–F Increased immunoreactivity of LC3B (red) and TOMM20 (green) in the EC region after hypoxia. Blue, DAPI. G, H Quantification of LC3B and TOMM20 fluorescence intensity in DG and EC. Bar = 50 μm. I, J Plots of pixel intensity of LC3B and TOMM20. The levels of LC3B (K, L), activated caspase-3 and caspase-3 (M–O) in the hippocampus and cortex detected by western blotting. P Neurons in the DG observed with transmission electron microscopy. Q–U Positive FJB signals in DG and EC in each group after hypoxia. Bar = 50 μm, n = 5/group. **P < 0.01, and ***P < 0.001, all compared with controls (One-way ANOVA).
