Fig. 5: AOA reversed the succinate accumulation, oxidative stress, and iron stress induced by hypoxia treatment. | Cell Death Discovery

Fig. 5: AOA reversed the succinate accumulation, oxidative stress, and iron stress induced by hypoxia treatment.

From: Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice

Fig. 5

Decreased levels of (A, B) succinate, (C, D) DCF level, and (G, H) mito-SOX in the hippocampus and cortex due to AOA treatment. E, F Flow cytometry-based quantification of hippocampal mito-SOX level. I–N Decreased immunoreactivity of IRP1 (red) and DMT1 (green) in the DG region after AOA treatment. Blue, DAPI. O, P Changed levels of IRP1 and DMT1 in the EC and DG. Bar = 50 μm. Q–S Expression of IRP1 and FPN1 in the cortex and hippocampus estimated using western blotting. n = 5/group. T, U Levels of iron (n = 5 per time point). *P < 0.05, **P < 0.01, and ***P < 0.001, all compared with hypoxia+saline group (One-way ANOVA).

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