Fig. 8: AICAR reversed the increased mitophagy level, neuronal injury, learning and memory deficits induced by hypoxia treatment.
From: Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice
A–F Decreased immunoreactivity of LC3B (red) and TOMM20 (green) in the EC region after AICAR treatment. Blue, DAPI. G, H Quantification of LC3B and TOMM20 fluorescence intensity in DG and EC. I–K The expression of activated caspase-3 and caspase-3 in hippocampus and cortex detected by western blotting. L–P Positive FJB signals in DG and EC in each group. Bar = 50 μm, n = 5/group. Q Latency to the platform (Two-way ANOVA). R Frequency of platform crossing. S Target quadrant time (%). T Opposite quadrant time (%). U Representative tracking. *P < 0.05, **P < 0.01, and ***P < 0.001, all compared with controls (One-way ANOVA). #P < 0.05, compared with that in the hypoxia+saline group (One-way ANOVA with Dunnett’s T3 post-hoc test)).
