Fig. 5: MAML3 is the target gene of miR-411-5p and promotes GC progression.
From: Hsa_circ_0007967 promotes gastric cancer proliferation through the miR-411-5p/MAML3 axis
A, B level of selected genes in BGC823 and SGC7901 cells transfected with miR-411-5p mimics. C The potential binding site between miR-411-5p and MAML3 mRNA. D The relative luciferase activity in HEK-293T cells cotransfected with miR-411-5p mimics and MAML3 plasmids with WT/MUT binding site by Dual luciferase reporter assays. E, F The expression of MAML3 in BGC823 and SGC7901 cells transfected with sinc, si-MAML3#1 and si-MAML3#2 by qPCR. G The expression of MAML3 in BGC823 and SGC7901 cells transfected with sinc, si-MAML3#1 and si-MAML3#2 by WB. H, I The growth rate of BGC823 and SGC7901 cells transfected with sinc, si-MAML3#1, siMAML3#2 by cck8 assays. J–L DNA positive cells of BGC823 and SGC7901 cells transfected with sinc, si-MAML3#1, siMAML3#2 by EdU assays. M, N The assessment of proliferation of BGC823 and SGC7901 cells transfected with sinc, si-MAML3#1 and si-MAML3#2 by colony formation assays. O The protein level of MAML3 of BGC823 and SGC7901 cells transfected with vector, hsa_circ_0007967 plasmids, miR-411-5p mimics or cotransfected with hsa_circ_0007967 plasmids and miR-411-5p mimics by WB.
