Fig. 4: EIF4EBP1 promoter activity is regulated by MYCN. | Cell Death Discovery

Fig. 4: EIF4EBP1 promoter activity is regulated by MYCN.

From: EIF4EBP1 is transcriptionally upregulated by MYCN and associates with poor prognosis in neuroblastoma

Fig. 4

a ChIP peaks of MYCN in the EIF4EBP1 promoter region in Kelly NB cell line. b Scheme of the EIF4EBP1 promoter reporter highlighting the three E-boxes corresponding to MYCN binding sites. c HEK-293-T cells were transfected with the wildtype EIF4EBP1 promoter firefly luciferase construct and with the indicated amounts of MYCN expressing plasmid (pMYCN). A Renilla Luciferase vector was used as an internal control. d MYCN and 4EBP1 protein expression were monitored in cell lysates from c by immunoblot analyses using the indicated antibodies. e HEK-293-T were transfected with wildtype or different E-box mutants EIF4EBP1 promoter firefly luciferase constructs with or without a MYCN expressing plasmid (pMYCN). A Renilla Luciferase vector was used as an internal control. Statistics were determined using Student’s t test or Mann–Whitney U-test. Exact p-values are presented. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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