Fig. 3: Effects of Rho and LIMK inhibition on cytoskeleton rearrangement and RhoA/LIMK/Cofilin pathway in FMNL2-silencing breast cancer cells. | Cell Death Discovery

Fig. 3: Effects of Rho and LIMK inhibition on cytoskeleton rearrangement and RhoA/LIMK/Cofilin pathway in FMNL2-silencing breast cancer cells.

From: FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway

Fig. 3

After transfection, MDA-MB-231 and BT549 cells were incubated with ZOL (100 or 70 μM) or BMS3 (20 or 10 μM) for 24 h, respectively, and then performed for indicated assays. A Representative fluorescent images of F-actin cytoskeleton were displayed. Scale bar, 50 μm. B The levels of FMNL2, Vimentin and Snail were measured by western blotting. C The levels of RhoA/LIMK/Cofilin-related molecules were examined by western blotting. D Co-IP analysis was used to determine the relationship between FMNL2 and RhoA. E The activity levels of RhoA were measured. *P < 0.05 versus NC siRNA group, #P < 0.05 versus siFMNL2 group.

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