Fig. 3: SOX5 inhibits HUVEC angiogenesis and hFOB1.19 cell osteogenesis by promoting EZH2 expression. | Cell Death Discovery

Fig. 3: SOX5 inhibits HUVEC angiogenesis and hFOB1.19 cell osteogenesis by promoting EZH2 expression.

From: Pro-angiognetic and pro-osteogenic effects of human umbilical cord mesenchymal stem cell-derived exosomal miR-21-5p in osteonecrosis of the femoral head

Fig. 3

A The transduction efficiency of SOX5 and EZH2 in cells validated with RT-qPCR; B immunoblotting analysis of the expression of SOX5 and EZH2 cells; C immunoblotting analysis of the expression of osteogenesis-related markers (OCN, Runx2, and Collagen I) in hFOB1.19 cells; D quantitative analysis of ALP activity of hFOB1.19 cells; E quantitative analysis of calcium deposition of hFOB1.19 cells by alizarin red S staining; F the migration of HUVECs determined with Transwell assay; G the tube-forming ability of HUVECs with total network area, total length, and the number of branch points determined with capillary-like tube formation assay. Cell experiment was repeated three times independently. *p < 0.05.

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