Fig. 1: Antiproliferative effect of iPA in GBM cells.
From: N6-isopentenyladenosine induces cell death through necroptosis in human glioblastoma cells
A Western Blot assay showing the different expression levels of EGFR in U87MG, U87 EGFRvIII, U87 EGFRwt, and NHA cell lines. B Graphic representation of results from MTT assays to determine cell viability of U87MG, U87EGFRvIII, and U87EGFRwt treated with different concentrations (0–10 µM) of iPA for 24 h and 48 h. C Antiproliferative effect of iPA on U87MG, U87 EGFRvIII, U87 EGFRwt GBM cell lines, and NHA cells as detected by BrdU assay after 24 h and 48 h of treatment. D Graphic representation of cell cycle distribution obtained using PI staining and flow cytometry. Data points are the percentage of cells in G0/1, S, and G2/M in U87MG at 24 h and 48 h after iPA treatment. The data are presented as mean ± SD of at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control, represented by cells treated with vehicle (DMSO).
