Fig. 3: iPA induces necroptosis.
From: N6-isopentenyladenosine induces cell death through necroptosis in human glioblastoma cells
A Plot showing the reduced ATP levels after 24 h of treatment with iPA 10 µM in U87 GBM stabilized cell lines. B, C Plot showing the % of PI-positive cells. Stabilized GBM cell lines were pretreated for 1 h with Necrosulfonamide (NSA) (5 μM) (B) and Necrostatin-1 (Nec-1) (20 μM) (C) and then analyzed through PI staining after 24 h of iPA treatment (10 μM). D Plot showing the % of cell viability in U87 GBM cell lines after 48 h of treatment with iPA (10 μM), RIP1 inhibitor Nec-1 (20 μM), Caspase inhibitor z-VAD (10 μM), and two combinations of these drugs: z-VAD (10 μM) + iPA (10 μM) and Nec-1 (20 μM) + iPA (10 μM). Specifically, GBM cells were pretreated with z-VAD or Nec-1 for 1 h, followed by treatment with iPA 10 μM for 48 h. The data are presented as mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control, represented by cells treated with vehicle (DMSO).
