Table 3 List of antibodies used.
From: N6-isopentenyladenosine induces cell death through necroptosis in human glioblastoma cells
Primary antibodies | Company | Dilution |
|---|---|---|
β-Actin | Santa Cruz Biotechnology (sc-47778); monoclonal anti-mouse antibody | 1:1000 |
Phospho-MLKL | Abcam (ab187091); monoclonal anti-rabbit antibody; Ser358 phosphorylated | 1:1000 |
MLKL | Santa Cruz Biotechnology (sc-293201); monoclonal anti-mouse antibody | 1:1000 |
Phospho-RIP1 | Cell Signaling (#65746); monoclonal anti-rabbit antibody; Ser166 phosphorylated | 1:1000 |
RIP1 | Santa Cruz Biotechnology (sc-133102); monoclonal anti-mouse antibody | 1:1000 |
Phospho-RIP3 | Abcam (ab 209384); monoclonal anti-rabbit antibody; Ser227 phosphorylated | 1:1000 |
RIP3 | Santa Cruz Biotechnology (sc-374639); monoclonal anti-mouse antibody | 1:1000 |
HMGB1 | Abcam (ab77302); monoclonal anti-rabbit antibody | 1:1000 |
PUMA | Santa Cruz Biotechnology (sc-377015); monoclonal anti-mouse antibody | 1:1000 |
EGFR | Cell Signaling (#4405 S); monoclonal anti-rabbit antibody | 1:1000 |
Caspase-3 | Cell Signaling (#9662); monoclonal anti-rabbit antibody | 1:1000 |
Caspase-8 | Santa Cruz Biotechnology (sc-6136); polyclonal anti-goat antibody | 1:1000 |
PARP | Cell Signaling (#9532); monoclonal anti-rabbit antibody | 1:1000 |
Secondary antibodies | Company | Dilution |
|---|---|---|
Goat Anti-Rabbit HRP | Bio Rad | 1:5000 |
Goat Anti-Mouse HRP | Bio Rad | 1:5000 |
