Fig. 5: E2F1 is a transcription factor of NOP. | Cell Death Discovery

Fig. 5: E2F1 is a transcription factor of NOP.

From: The nociceptin receptor promotes autophagy through NF-kB signaling and is transcriptionally regulated by E2F1 in HCC

Fig. 5

A Correlations between NOP expression and the expression of candidate transcription factors. B Expression of E2F1 and MAZ in HCC and adjacent tissues. C Correlation between NOP expression and E2F1 or MAZ expression. D IB analysis of the indicated proteins in MHCC-LM3 and Huh7 cells. E CCK-8 assay showing the proliferation of vector + shNC, vector + shNOP, E2F1 + shNC, and E2F1-shNOP HCC cells. F Apoptosis rates in vector + shNC, vector + shNOP, E2F1 + shNC, and E2F1-shNOP HCC cells. G CCK-8 assay showing the proliferation of shNC + vector, shNC + NOP, shE2F1 + vector, and shE2F1 + shNOP HCC cells. H Apoptosis rates in shNC + vector, shNC + NOP, shE2F1 + vector, and shE2F1 + shNOP HCC cells. I IB analysis of the indicated proteins associated with autophagy and apoptosis in MHCC-LM3 and Huh7 cells. J Schematic drawing of the full-length and truncated NOP fragments. K Luciferase assay showing the binding site of NOP and E2F1. L. A specific band of the expected size was detected in the input DNA and the anti-E2F1 antibody (anti-E2F1)-precipitated DNA by ChIP. M Schematic showing the effect of increased expression of NOP via the transcription factor E2F1. ***P < 0.001, *P < 0.05.

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