Fig. 1: Localization of native BKCa channel in neonatal and cardiomyocytes isolated from the rodent heart. | Cell Death Discovery

Fig. 1: Localization of native BKCa channel in neonatal and cardiomyocytes isolated from the rodent heart.

From: Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury

Fig. 1

A, B, C, and D Isolated cardiomyocytes loaded with WGA (magenta), fixed, permeabilized, and labeled with BKCa (green), and ATP5A (red), antibodies. Nuclei were stained with DAPI (blue). The right panels in A, B, C, and D show merged images at higher magnification. E The purple bar represents percentage colocalization between the BKCa channel and plasma membrane (WGA), which is significantly higher in neonatal cardiomyocytes compared to adult cardiomyocytes. The red bar represents percentage colocalization between the BKCa channel and mitochondria, which is lower in neonatal cardiomyocytes as compared to adult cardiomyocytes isolated from rodent hearts. All experiments were repeated independently at least four times and colocalization data are represented as mean ± SEM from 10 cells in each “n” number. P-values were determined by a one-tailed paired student’s t-test; **≤ 0.001, ***≤ 0.0001, ###≤ 1.0*10–8.

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