Fig. 3: Mitochondrial targeting BKDEC splicing increases with age in neonatal cardiomyocytes. | Cell Death Discovery

Fig. 3: Mitochondrial targeting BKDEC splicing increases with age in neonatal cardiomyocytes.

From: Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury

Fig. 3

A–F Cardiomyocytes isolated from aging (P3, P7, P14, P21, P28, and adult) rats loaded with WGA (magenta), fixed, permeabilized, and labeled with BKCa (green) and ATP5A (red). The nucleus was stained with DAPI (blue). The right panels of A–F show colocalization of merged images between BKCa to WGA and BKCa to ATP5A at higher magnification. G Protein proximity index analysis showing percentage colocalization of BKCa to WGA decreases, whereas BKCa to mitochondria increases in cardiomyocytes isolated from P3 to the adult stage. H Relative levels of BKCa FL and BKCa DEC mRNA expression in cardiomyocytes isolated from P3, P7, P14, P21, P28, and adult hearts were measured by qRT-PCR and were normalized to their relative GAPDH expression. Data represented as fold-change to P3 BKCa FL and P3 BKCa DEC; mean ± SEM; at least three independent experiments. P-values were determined by a one-tailed paired student’s t-test; *≤ 0.05, **≤ 0.001, ***≤ 0.0001, ****≤ 0.00001.

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