Fig. 1: EDN1 did not cause RGC death directly or via macroglia-expressed receptors. | Cell Death Discovery

Fig. 1: EDN1 did not cause RGC death directly or via macroglia-expressed receptors.

From: Vascular derived endothelin receptor A controls endothelin-induced retinal ganglion cell death

Fig. 1

A Fluorescein angiography of retinal vasculature in naive and EDN1-injected eyes from WT, Six3-cre+Ednrafl/flEdnrb+/+, Six3-cre+Ednra+/+Ednrbfl/fl, and Six3-cre+Ednrafl/flEdnrbfl/fl animals. Deletion of either or both Edn receptors with Six3-cre did not prevent EDN1-induced vasoconstriction (n ≥ 3). B Retinal flat mounts and quantification of cleaved caspase 3+ (cCASP3+, red) RBPMS+ (green) cells from WT, Six3-cre+Ednra+/+Ednrbfl/fl, Six3-cre+Ednrafl/flEdnrb+/+, Six3-cre+Ednrafl/flEdnrbfl/fl 5 days post-EDN1 or PBS (vehicle control) injection. Each genotype group had significant increases in cCASP3+ RGCs compared to PBS controls. No significant difference in cCASP3+ RGCs was observed between genotype groups after EDN1. cCASP3+ RGCs/mm2 ± SEM: PBS: 0.8 ± 0.3, WT: 32.0 ± 7.2, Six3-cre+Ednrafl/flEdnrb+/+: 23.7 ± 8.6, Six3-cre+Ednra+/+Ednrbfl/fl: 32.3 ± 9.5, Six3-cre+Ednrafl/flEdnrbfl/fl: 19.8 ± 6.0 (n ≥ 7 per genotype, *P < 0.05, Kruskal–Wallis test). Scale bars, 50 μm.

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