Fig. 2: Cag-creERT2 robustly recombined floxed alleles in retinal and optic nerve DAPI+ cells. | Cell Death Discovery

Fig. 2: Cag-creERT2 robustly recombined floxed alleles in retinal and optic nerve DAPI+ cells.

From: Vascular derived endothelin receptor A controls endothelin-induced retinal ganglion cell death

Fig. 2

A Cag-creERT2+Tdtomato+ retinal and optic nerve head sections depicting cell-type-specific expression of Tdtomato. Cag-creERT2 robustly recombined floxed alleles in DAPI+ cells, including RBPMS+ RGCs, SOX2+ Müller glia, GFAP+ retinal astrocytes, CD31+ vascular cells, and SOX2+ GFAP+ optic nerve head (ONH) astrocytes (n = 3). Scale bars, 50 μm. B Fluorescein angiography of retinal vasculature in naive and EDN1-injected eyes from WT, Cag-creERT2+Ednrbfl/fl, and Cag-creERT2+Ednrafl/fl animals. Full body deletion of Ednra ablated EDN1-induced vasoconstriction (n ≥ 5).

Back to article page