Fig. 4: Myh11-creERT2 recombined floxed alleles in vascular mural cells.
From: Vascular derived endothelin receptor A controls endothelin-induced retinal ganglion cell death
A Myh11-creERT2+Tdtomato+ fluorescein angiography overlayed with TdTomato fluorescence demonstrating TdTomato localization to retinal arteries (n = 4). B Myh11-creERT2Tdtomato+ retinal flat mounts counterstained with CD31 to visualize retinal vasculature. Tdtomato was robustly and specifically expressed by arterial cells and capillaries, but not by RBPMS+ RGCs or any other observable cell type. C Tdtomato+ cells surrounding retinal capillaries were apparent in superficial, intermediate, and deep layers of the retina (n = 3). Scale bars, 50 μm. D Fluorescein angiography of retinal vasculature in naive and EDN1-injected eyes from WT and Myh11-creERT2+Ednrafl/fl animals. Mural cell-specific deletion of Ednra ablated EDN1-induced vasoconstriction (n ≥ 5).
