Fig. 5: EDNRA expressed by vascular mural cells elicited RGC death in response to EDN1. | Cell Death Discovery

Fig. 5: EDNRA expressed by vascular mural cells elicited RGC death in response to EDN1.

From: Vascular derived endothelin receptor A controls endothelin-induced retinal ganglion cell death

Fig. 5

A Flat mounted retinas immunoassayed for cCASP3 and RBPMS 5 days post-EDN1 injection. Ednra deletion from vascular mural cells significantly reduced numbers of cCASP3+ RGCs after EDN1 injury. cCASP3+ RGCs/mm2: WT: 23.6 ± 7.6, Myh11-creERT2+Ednrafl/fl: 6.0 ± 4.4 (n ≥ 9, *P = 0.009, Mann–Whitney test). B Flat mounted WT and Myh11-creERT2+Ednrafl/fl retinas immunoassayed for RBPMS 28 days post-EDN1 injection. Mural cell deletion of Ednra prevented EDN-induced RGC loss. %RBPMS+ cell survival ± SEM for WT and Myh11-creERT2+Ednrafl/fl respectively: PBS: 100.0 ± 2.3, 100.0 ± 1.4; EDN1: 85.0 ± 4.3, 101.4 ± 1.3 (n ≥ 8, *P ≤ 0.001, two-way ANOVA, Holm–Sidak post hoc). Scale bars, 50 μm.

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