Fig. 7: JAC1 activates JWA expression by inhibiting YY1 through the ubiquitin–proteasome pathway. | Cell Death Discovery

Fig. 7: JAC1 activates JWA expression by inhibiting YY1 through the ubiquitin–proteasome pathway.

From: JAC1 targets YY1 mediated JWA/p38 MAPK signaling to inhibit proliferation and induce apoptosis in TNBC

Fig. 7

A Western bolt assay analyzed the YY1 expression in nucleoplasm and cytoplasm treated with increasing doses of JAC1 in both MDA-MB-231 and SUM1315 cells. B YY1 stability assay. MDA-MB-231 cells were treated by JAC1 for 24 h and followed by exposed to CHX for indicated time; expression of YY1 was determined by Western blot. C The time-course intensities of the YY1 protein after treated by JAC1 and followed by indicated time of CHX exposed. D Immunofluorescence analyzed the expressions and location of YY1 in SUM1315 cells after treated with JAC1 at indicated doses (5 μM). JAC1 increases ubiquitination of YY1. The MDA-MB-231 (E) and SUM1315 (F) cells were treated with JAC1, followed by MG132; ubiquitination and expression of YY1 were determined by Co-IP and Western blot, respectively. G The expression levels of YY1 in MCF10A, MDA-MB-231, and SUM1315 cells. H Kaplan–Meier curves depicted survival according to the expression of YY1 in the TNBC cohort from TCGA database. P values were calculated with the log-rank test. I Schematic diagram of the molecular mechanism of JAC1 inhibiting the proliferation of TNBC.

Back to article page