Fig. 1: POL regulates the progression of OL to POL by secreting exosomes. | Cell Death Discovery

Fig. 1: POL regulates the progression of OL to POL by secreting exosomes.

From: Low-metastatic melanoma cells acquire enhanced metastatic capability via exosomal transfer of miR-199a-1-5p from highly metastatic melanoma cells

Fig. 1

A The change of migration ability of OL cells after co-culture with POL cells, bar = 60 μm. B Quantitative assessment of the change of migration ability of OL cells after co-culture with POL cells, ***P < 0.001. TEM analysis (bar = 100 nm, C), NTA analysis (D) and WB analysis of protein markers (E) of exosomes secreted by OL and POL cells. F Imaging analysis of the time course of POL-exo uptake by OL cells, bar = 10 μm. Visualization (G, bar = 60 μm) and quantitative analysis (H) of the effect of exosomes secreted by POL (POL-exo) on the migration ability of OL. I The dose effect of POL-exo on migration ability of OL. Visualization (J, bar = 60 μm) and quantitative analysis (K, ***P < 0.001) of the effect of exosomes secreted by POL (POL-exo) on the invasiveness of OL cells. Visualization (L) and quantitative analysis (M, **P < 0.01) of the effect of exosomes secreted by POL (POL-exo) on the clonogenic proliferation of OL cells. N RAB27A mRNA expression (N, **P < 0.01) and protein expression (O) in POL-N.C.and POL-siRAB27A. Visualization (P, bar = 60 μm) and quantitative analysis (Q, ***P < 0.001) of the effect of siRAB7A on the invasiveness of OL cells.

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