Fig. 5: HECTD3 interacts with c-MYC and mediates K29 site-linked polyubiquitination of c-MYC.
From: HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC
A The proximity ligation (PLA) experiments were applied to assess the interaction of HECTD3 and c-MYC. B DOC and HECT domains diagram of HECTD3. C TAD, CP, and bHLHZ domains diagram of c-MYC. D, E Interaction between c-MYC and HECTD3 truncated domains in 293FT cells. F 293FT cells were cotransfected with c-MYC, HA-Ub, and increasing amounts of HECTD3 plasmids and treated with MG132 were immunoprecipitated using PARP1 beads and immunoblotted using an anti-HA antibody. G The Flag-HECTD3, c-MYC, HA-Ub, and ubiquitin mutant plasmid were cotransfected and treated with MG132 for ubiquitination assays in 293FT cells. H The Flag-HECTD3, c-MYC, HA-Ub, and C823A were cotransferred and treated with MG132 for ubiquitination assay in 293FT cells.
