Fig. 2: Effect of MFG-E8 in H2O2-induced ECM degeneration in rat NP cells. | Cell Death Discovery

Fig. 2: Effect of MFG-E8 in H2O2-induced ECM degeneration in rat NP cells.

From: MFG-E8 alleviates intervertebral disc degeneration by suppressing pyroptosis and extracellular matrix degradation in nucleus pulposus cells via Nrf2/TXNIP/NLRP3 axis

Fig. 2

A, B Effects of MFG-E8 on the proliferation of NP cells with or without H2O2 exposure were measured by CCK-8 assays. C, D The protein expressions of Collagen II, Aggrecan, MMP13, and ADAMTS5 in NP cells treated as above were visualized by western blot. E The expression of Collagen II and Aggrecan was assessed by immunofluorescence. All data are presented as mean ± standard deviation (SD), n = 6; *p < 0.01 vs. untreated group, **p < 0.01 vs. untreated group, #p < 0.05 vs. H2O2 group, ##p < 0.01 vs. H2O2 group.

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