Fig. 3: Effect of MFG-E8 in H2O2-medicated pyroptosis in rat NP cells. | Cell Death Discovery

Fig. 3: Effect of MFG-E8 in H2O2-medicated pyroptosis in rat NP cells.

From: MFG-E8 alleviates intervertebral disc degeneration by suppressing pyroptosis and extracellular matrix degradation in nucleus pulposus cells via Nrf2/TXNIP/NLRP3 axis

Fig. 3

A, B Western blot and its quantification revealed the expression level of NLRP3, C-Caspase-1, GSDMD-N, IL-1β, and IL-18. C Effect of MFG-E8 on H2O2-exposed IL-1β production in NP cells was detected by ELISA. D the LDH release in NP cells. E, F Calcein-AM/PI confirmed the death level of H2O2-stimulated Rat NP cells. All data are presented as mean ± standard deviation (SD), n = 6; **p < 0.01 vs. untreated group, ##p < 0.01 vs. H2O2 group.

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