Fig. 8: MFG-E8 upregulates Nrf2 activation through the PI3K/AKT signaling pathway in rat NP cells. | Cell Death Discovery

Fig. 8: MFG-E8 upregulates Nrf2 activation through the PI3K/AKT signaling pathway in rat NP cells.

From: MFG-E8 alleviates intervertebral disc degeneration by suppressing pyroptosis and extracellular matrix degradation in nucleus pulposus cells via Nrf2/TXNIP/NLRP3 axis

Fig. 8

A–C Western blot and its quantification revealed the protein expressions of p-PI3K, PI3K, p-Akt, and Akt in NP cells after being treated with or without the administration of MFG-E8 with H2O2. D–F We evaluated the level of Nrf2 in the nucleus and the level of p-AKT and AKT in the whole cells by western blot after in after NP cells after treatment with H2O2 or MFG-E8 or LY294002. G The Nrf2 was detected by immunofluorescence combined with DAPI staining for nuclei. All data are presented as mean ± standard deviation (SD) of, n = 6; *p < 0.05 vs. untreated group, ##p < 0.01 vs. H2O2 group, &&p < 0.01 vs. H2O2 + MFG-E8 group.

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