Fig. 2: Overexpression of FTO inhibits the progression of PCa in vitro. | Cell Death Discovery

Fig. 2: Overexpression of FTO inhibits the progression of PCa in vitro.

From: N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability

Fig. 2

A Western blot and RT-qPCR showed the overexpression efficiency of FTO plasmid and control vector in DU145 and C4-2 cell lines. GAPDH was the internal reference. B CCK-8 proliferation assay, C EdU assay, and (D) Colony-formation assay showed that up-regulating FTO inhibits PCa cells proliferation. F Transwell assay and (G) Wound healing assay suggested that overexpression of FTO significantly inhibits cells migration in DU145 and C4-2 cells. E, H Graphical illustration of statistical results of transfection of FTO plasmid or control Vector on cell proliferation and migration. *P < 0.05; ***P < 0.001; ****P < 0.0001; t-test. Error bars, mean ± SD (n = 3).

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