Fig. 2: The extracellular domain and the cytoplasmic tail are essential in the antiapoptotic effect of MUC21.
From: Mucin 21 confers resistance to apoptosis in an O-glycosylation-dependent manner
a Flow cytometric analysis of two clonal populations of HEK293 transfectants with MUC21 lacking the tandem- repeat domain with rabbit anti-CT polyclonal antibody (Anti-CT) after permeabilization. b Flow cytometric analysis of two clonal populations of HEK293 transfectants with MUC21 lacking the cytoplasmic tail domain with a 1:1 mixture of mAbs heM21C (21C) and heM21D (21D). The solid line represents antibody binding and the shaded area represents isotope control antibody binding. c Western blotting analysis of electrophoretically separated lysates of mock-transfected cells and two clones of transfectants with MUC21 lacking the tandem- repeat domain (Δ-TR-1 and Δ-TR-2, left panel), and mock-transfected cells and two clones of transfectants with MUC21 lacking the cytoplasmic tail (Δ-CT-1 and Δ-CT-2, right panel). d Percentage of mock transfectants, two clones of transfectants with MUC21 lacking the tandem-repeat domain (Δ-TR-1 and Δ-TR-2) and two clones of transfectants with MUC21 lacking the cytoplasmic tail (Δ-CT-1 and Δ-CT-2) undergoing apoptosis after treatment with 100 µM etoposide for 48 h. Four independent experiments are shown. The mean is indicated as a horizontal line. Two-way ANOVA with Tukey’s multiple-comparison test. **p < 0.01, n.s. not significant.
