Fig. 4: LINC00857 sponges with miR-130b. | Cell Death Discovery

Fig. 4: LINC00857 sponges with miR-130b.

From: Long noncoding RNA LINC00857 promotes pancreatic cancer proliferation and metastasis by regulating the miR-130b/RHOA axis

Fig. 4

A A RIP assay was performed to assess the LINC00857 level in PC cells transfected with Ago2 overexpression vectors or control vectors. B Schematic illustration showing the overlapping target miRNAs of LINC00857 predicted by DIANA, LINC editing, and RNA22. C An RNA pull-down assay was performed to determine the binding between LINC00857 and target miRNAs. D, E The expression of miR-130b, miR-148b, and miR-106b in PC cells with LINC00857 overexpression and knockdown. F The binding site between LINC00857 and miR-130b. G Luciferase activity was analyzed in PC cells after transfected LINC00857 wt or LINC00857 mut and miR-130b mimics or NC mimics. H The miR-130b expression was evaluated in PC tissues and adjacent tissues. I The co-expression relationship between miR-130b and LINC00857 was determined by qRT-PCR. *P < 0.05, **P < 0.01.

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