Fig. 6: LINC00857 positively regulates RHOA expression in PC cells by sponging miR-130b. | Cell Death Discovery

Fig. 6: LINC00857 positively regulates RHOA expression in PC cells by sponging miR-130b.

From: Long noncoding RNA LINC00857 promotes pancreatic cancer proliferation and metastasis by regulating the miR-130b/RHOA axis

Fig. 6

A Pathway analysis showed that pathways which LINC00857 co-expressed genes enriched in. B GSEA analysis demonstrated that LINC00857 significantly and positively associated with RHO pathway activation. C Schematic illustration showing the overlapping target genes of miR-130b predicted by miRDB, TargetScan, miRcode and gene co-expression of LINC00857. D The binding site between miR-130b and RHOA is shown. E Dual-luciferase activity was analyzed in PC cells after transfected RHOA wt or RHOA mut and miR-130b mimics or NC mimics. F RHOA mRNA expression levels in PC cells with LINC00857 overexpression and knockdown were evaluated by qRT-PCR. G Western blot was performed to determine RHOA and ROCK protein expression in PC cell with LINC00857 overexpression and knockdown. H, I Based on the TCGA database, it was demonstrated that RHOA was upregulated in PC tissues, while PC patients with high RHOA expression had a low disease-free survival rate. J The mRNA level of RHOA in PC tissues and adjacent tissues is shown. K The co-expression relationship between LINC00857 and RHOA is shown. L The co-expression relationship between miR-130b and RHOA is shown. *P < 0.05, **P < 0.01.

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